CYTOTOXIC EFFECT OF MANGROVE EXTRACT FROM AVICENNIA MARINA LEAVES ON STAPHYLOCOCCUS SP

Abstract

INTRODUCTION:

Staphylococcus sp are a group of Gram Positive bacteria that commonly reside on human skin and mucous membranes.Staphylococcus sp have emerged as significant pathogens, causing a wide range of infections, from mild skin infections to life threatening diseases like endocarditis. Avicennia marina, commonly known as gray mangrove or white mangrove, is a species of mangrove tree classified in the plant family Acanthaceae. The objective of this study is to investigate the cytotoxic effect of mangrove extract against Staphylococcus sp and This study aims to Evaluate the cytotoxic effect of Avicennia marina extract against Staphylococcus sp focusing on its potential antimicrobial activity.

MATERIALS AND METHODS:

Clinical isolates of Staphylococcus species were obtained and stored appropriately. Avicennia marina leaves were collected, air-dried, and extracted with methanol. Staphylococcus sp. Biofilms were formed on Baird Parker broth base using coverslip method.Biofilms were stained with acridine orange and propidium iodide. Cell viability analysis was conducted using the MTT assay after exposing the Staphylococcus sp to Avicennia marina extract. The extract was administered every 24 hours for 96 hours to evaluate antimicrobial activity through changes in biofilm staining and confocal imaging analysis.

RESULTS:

The extract of Avicennia marina demonstrated remarkable inhibitory effects on the growth of Staphylococcus species. When the bacteria were treated with 1 ml/mg of the extract every 24 hours for a total duration of 96 hours, the extract consistently exhibited the ability to suppress the growth of Staphylococcus, indicating its potential as a potent natural antimicrobial agent.

CONCLUSION:

In conclusion, the extract consistently showed the ability to suppress the growth of Staphylococcus, suggesting its potential as a natural antimicrobial agent. These findings highlight the promising antimicrobial properties of Avicennia marina extract, which may have significant implications for the development of novel therapeutics to combat Staphylococcus infection.