ANTIBACTERIAL ACTIVITY OF ETHANOLIC AND METHANOLIC SEED EXTRACT OF FIVE CASSIA SPECIES AGAINST SOME PATHOGENIC BACTERIA
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Abstract
Current investigation was accomplished at biology department/faculty of science/kufa university during 2023-2024. Seeds organic solvent extract was prepared using methanol and ethanol by Soxhlet) at three concentration (100mg/ml/,200mg/ml and 400 mg/ml) for five Cassia species (1-C. fistula, 2-C.bakeriana, 3-C.gluaca , 4-C.siamea 5-C.alata ) . examined bacterial strain were resistance to many antibiotics. slight variation observed between cassia species and between methanol and ethanol extract, high concentration produced highest bacterial growth inhibition. E.coli gave highest growth inhibition zone compared to S. aureus and K. pneumoniae . Both methanolic and ethanolic extract of examined Cassia species possess antibacterial activity against examined bacterial strains especially with increasing concentration compared to control (DMSO) treatment with exception of C.fistula methanolic extract at concentration of 100mg/ml against S. aureus and C.alata ethanolic extract at concentration of 100mg/ml against K. pneumonia ,both failed to give any antibacterial activity. C.siamea methanolic extract at concentration of 400mg/ml gave highest antibacterial activity against E.coli.