PREPARATION OF TERMINALIA CHEBALA ETHANOLIC EXTRACT BASED MOUTHWASH AND ITS ANTIMICROBIAL AND CYTOTOXICITY EFFECT

Abstract

Antimicrobial activity is the process of killing or inhibiting the disease causing microorganisms. Many antimicrobial agents are used for this purpose. The effect of being harmful to cells caused by toxic agents is called cytotoxicity. Exposing cells to a cytotoxic agent may result in various outcomes in the functional and structural ability of cell. No challenges are faced in previous research. The research is needed to find whether the ethanolic extract of terminalia chebula extract mouthwash has antimicrobial activity and cytotoxicity. The research also fulfils the deficiency of work on comparing its antimicrobial activity on different families.The aim of the research is to find the antimicrobial and cytotoxicity effect of ethanolic extract of terminalia chebula extract mouthwash. For cytotoxicity effect, a total of 6 wells with salt water and 10 nauplii is taken and one well is controlled and the others are filled with 5, 10,20,40,80????l concentration of prepared mouthwash. left for 24hrs and the alive nauphlii in each. For antimicrobial activity, 25 , 50, 100 ????l of prepared mouthwash and a  mouthwash already available in the market are taken in the culture medium of 4 different microorganisms. and left for 24 hrs.the  ethanolic extract mouthwash of t.chebula has higher antimicrobial activity than the mouthwash present in the market. And also has less cytotoxicity in its less concentration.