IMPROVING THE PHARMACEUTICAL EFFECT OF AMPICILLIN BY TRIGONELLA FOENUM-GRAECUM (TFG) NANOCOMPOSITE OF RESISTANT BACTERIA S.AUREUS AND E.COLI CAUSING RECURRENT MISCARRIAGE ISOLATED FROM WOMEN WITH UTIs

Main Article Content

Zianb Adwan Toama, Heyam Abdul Ridha Al-awad

Abstract

Abstract

The current study aimed to isolate and diagnose the bacteria that cause urinary tract infections in pregnant women and cause miscarriage, estimate the resistance of the isolated bacterial species, and evaluate the inhibitory effect of Trigonella foenum-graecum extract. (TGF) and the nanocomposite prepared in green ways from the seeds of the fenugreek plant TGF/Nps before and after loading the antibiotic Ampiciliin into the bacteria E. coli and S. aureus, which are most common in pregnant women and aborted women with urinary tract infections. The results of the sensitivity test showed that most of the isolates were bacteria. E. coli and S. aureus were resistant to most antibiotics and sensitive to others. They were diagnosed using the VITEK 2 system test. The resistance of E. coli to the antibiotic (Ampicillin) was 66.66%, while the resistance of S. aureus to the same antibiotic reached 100% at the MIC concentration. The inhibitory effect on the growth of E. coli and S. aureus bacteria was studied by the TGF/NPs nanocomposite in its free form (T3) and the results were compared with the inhibitory ability of the free AMP antibiotic (T1) with what was recorded of the synergistic effect of the antibiotic after loading it. Treatment with the nanocomposite With the antibiotic TGFNps/AMP (5T), it showed the highest rate of inhibition at the first and second concentrations (C1,2 C). These increases in the rate of inhibition were significant (P≤0.05), as the rate of inhibition for the two concentrations compared to what was recorded in the fourth treatment. (T4), which treatment with the extract loaded with the antigen (TGF/AMP). To evaluate the inhibitory action of the isolated E. coli bacteria, the study recorded an increase in the diameter of the inhibition ring of the antibiotic (AMP) when loaded with the green nanocomposite of the plant The increase in TGFNps/AMP (T5/C1-2) was significant when compared with what was recorded in the results of treatment with the free antibiotic and the free nanocomposite at the two concentrations above. When treated with the first concentration (C1), the rate of inhibition of S. aureus in the treatment with the second concentration (C2), and the results were also high when compared with what was recorded in the fourth treatment with its first and second concentrations (T4/C1-2).

Article Details

How to Cite
Heyam Abdul Ridha Al-awad, Z. A. T. (2024). IMPROVING THE PHARMACEUTICAL EFFECT OF AMPICILLIN BY TRIGONELLA FOENUM-GRAECUM (TFG) NANOCOMPOSITE OF RESISTANT BACTERIA S.AUREUS AND E.COLI CAUSING RECURRENT MISCARRIAGE ISOLATED FROM WOMEN WITH UTIs. Obstetrics and Gynaecology Forum, 34(3s), 2485–2493. Retrieved from https://obstetricsandgynaecologyforum.com/index.php/ogf/article/view/733
Section
Articles