THE INTERACTION OF BACTERIAL BIOFILM AND SEAWEED EXTRACT AGAINST PATHOGEN Proteus mirabilis

Abstract

Introduction: Proteus mirabilis is a Gram negative bacterium that is a frequent cause of catheter-associated urinary tract infections (CAUTIs). Its ability to cause such infections is mostly related to the formation of biofilms on catheter surfaces. Nowadays, seaweeds are considered a novel source of bioactive compounds and produce a great variety of secondary metabolites exhibiting broad spectrum of biological activities. In this we consider the potentiality of seaweed - sargassum.

Aim & objectives: The present study aims to explore the antibacterial activity of sargassum seaweed extracts against Proteus mirabilis

Materials and methods: By examining its morphological description, existing literature references, and employing herbarium comparison studies, the Sargassum spp. identification was completed. Methanolic extract of sargassum was prepared. Antibacterial activity was studied using microscope and pcr analysis of virulence gene.

Results: The methanolic extract of sargassum has a maximum antibacterial activity of 68.2% at a concentration of 100 microgram/mL. The strongest peak in the FTIR analysis of Sargassum indicates that phenol is the main component in this seaweed extract.

Conclusion: In the present investigation methanolic extract of sargassum showed a promising antibacterial effect against the biofilm of Proteus mirabilis.