IN VIVO AND IN VITRO EFFECTS OF BIOSYNTHESIZED ZnNPs AND AgNPs USING SESAMUM INDICUM SEEDS AGAINST MDR ENTEROCOCCUS HIRAE ON WOUND HEALING IN ERBIL CITY/IRAQ

Abstract

Abstract

A problem facing modern medicine is wound infection caused by microorganisms that are resistant to drugs (MDR). To counter such a danger, new antibacterial agents like nanoparticles (NPs) are needed. The goal of this research is to identify a different treatment drug to fight the isolates of MDR Enterococcus hirae.

A total of 157 clinical specimens were obtained from various wounds and hospitals located in Erbil, Iraq, between July and October of 2022. 61 (38.21%) Gram positive bacteria (GPB) were found; based on 16S rRNA (PCR), sequencing, and the GenBank database, one (1.6%) of these was newly reported in Iraq. According to the findings of a sensitivity test conducted on 20 antimicrobials, E. hirae was resistant to 13 different antibiotics. Using an albino wistar rat model, zinc nanoparticles (ZnNPs) and silver nanoparticles (AgNPs) derived from Sesamum indicum seeds were biosynthesized and evaluated against isolated in vitro antibacterial and in vivo wound healing. In the current study, ZnNPs and AgNPs inhibition zones have 11 mm of antibacterial activity against E. hirae for each. With the standard therapies, the recovery period with ZnNPs and AgNPs creams was noticeably faster than that of usual therapy. Wounds infected with E. hirae that were treated with Zn NP, AgNPs, vaseline, fusidin, and mebo healed in 18, 16, 20, 24, and 21 days, respectively and the non-treated group healed in 39 days. The control (non-treated group) had slower healing times of wounds. The recent work is a significant step towards creating innovative nanoparticles that provide better alternative uses for antibacterial medications and wound treatments.