GREEN SYNTHESIS OF TERMINALIA ARJUNA-MEDIATED SELENIUM NANOPARTICLES: CHARACTERISATION AND IN-VITRO EVALUATION OF NANOGEL FOR ENHANCED WOUND HEALING

Abstract

Abstract

Introduction: This study aims to investigate the green synthesis of selenium nanoparticles (SeNPs) using Terminalia arjuna and assess their potential application in wound healing. The research explores the distinctive characteristics of SeNPs, such as their crystalline nature and complex composition, with the ultimate goal of contributing to advancements in nanomedicine and wound care.

Methods: X-ray diffraction (XRD) analysis confirmed the crystalline nature of SeNPs, revealing a nuanced structural complexity with both crystalline and amorphous components. Fourier-Transform Infrared (FTIR) Spectroscopy identified specific functional groups, further affirming SeNP formation. Scanning Electron Microscopy (SEM) showcased a prevalent nanoaggregate structure, suggesting potential applications in nanomedicine and catalysis.

Results: The calculated crystallite size of 30 nanometers, determined by the Debye-Scherrer equation, indicated a uniform size distribution within the SeNP ensemble. The SEM imaging highlighted the tendency of SeNPs to cluster, emphasizing their potential in specific applications. The synthesized SeNPs were incorporated into a gel, exhibiting higher viscosity than the control group. Gel dispersion studies indicated suitability for controlled and sustained delivery. Cytotoxicity assessment on Human periodontal ligament fibroblast cells revealed concentration-dependent reduction in cell viability, underscoring potential cytotoxic effects. Cell morphological analysis demonstrated distinct alterations induced by the T. arjuna SeNPs nanogel, including enhanced cell migration, filopodia formation, and a spindle-like appearance. These changes suggested a potential influence on key cellular processes. In-vitro scratch wound healing assays provided compelling evidence of the therapeutic efficacy of the T. arjuna SeNPs nanogel. Treated cells exhibited accelerated wound closure, emphasizing the gel's potential in promoting wound healing processes.

Conclusion: The study successfully achieved its aim by providing a comprehensive exploration of SeNP synthesis and gel formulation. The observed effects on cellular processes and wound healing underscore the significant promise of SeNPs as a therapeutic antibacterial platform. The multifaceted attributes of the synthesized SeNPs and their gel formulation present a promising avenue for future exploration in advanced wound care and nanomedicine.