EVALUATING THE ANTIMICROBIAL EFFICACY AND CYTOTOXICITY OF BIOACTIVE COMPOUND BASED NANOPARTICLES AS A ORAL RINSE: A COMPREHENSIVE STUDY

Abstract

Introduction: Research on nanotechnology has increased significantly in recent years, particularly in the areas of green synthesis and nanoparticle characterization. Pomegranate (Punica granatum) has been employed in several medical systems for its medicinal benefits, and one of its biological features has been identified as antibacterial activity. Green synthesis of AgNPs is shown to help in the formulation of potent antibacterial drugs for use against microorganisms that pose a threat to public health. This study aims to synthesize, characterize and assess the cytotoxic activity of Punica granatum mediated silver nanoparticles mouthrinse.

Materials and methods: 1 mm of Silver nitrate solution was prepared by dissolving 1 gram of silver nitrate powder in 90 ml of distilled water and is incorporated in 10 ml of PG extract. The combined solution was placed in magnetic orbital shaker for 24 hours. The solution was then centrifuged to obtain nanoparticle pellets. A mouthwash was prepared using the nanoparticles and its antimicrobial and antiplaque potential was assessed.

Results and Discussion: The Ag-PG nanoparticles showed a color change and its formation  was further  confirmed by  UV-Visible spectroscopy which  peaked at  350 nm. Different concentrations of nano-particles exhibited different levels of antimicrobial and anti plaque potential.

Conclusion: Thus, it can be concluded that the Punica granatum mediated silver nanoparticles based mouthwash that was formulated showed various spectrum of antimicrobial and anti plaque potential activity at different concentrations of nano-particles and is considered safe for use as a mouth rinse.

Keyword: Pomegranate, Mouthwash, Herbal Mouthwash, antimicrobial activity, Chlorhexidine alternatives, Silver Nanoparticles, Bioactive compound.