BIOSYNTHESIS OF SILVER NANOPARTICLES PRODUCED FROM PLEUROTUS OSTREATUS FUNGUS AGAINST STAPHYLOCOCCUS AUREUS ISOLATED FROM DIABETIC FOOT ULCERS

Abstract

Abstract:   

   Diabetic foot ulcers suffer from the risk of developing diabetic foot infections, as the development of bacterial resistance to antibiotics is a major problem that limits the use of antibiotics to treat diseases resulting from bacteria. From this perspective, our study aims to isolate and diagnose   diabetic foot ulcers. A total of 105 specimens were collected, there were two different types of diabetes  and type 1 diabetes. Their number reached 83 (79.04%),  patients, and type 2 diabetes occurred in 22 (20.95%),  out of 105 patients. The Staphylococcus aureus found naturally on the skin but can cause infection when it gets into wounds or ulcers it may be the cause of the infection diabetic foot ulcers .

  In this study, we examined the effectiveness of silver nanoparticles extracted and manufactured from the fungus , Pleurotus ostreatus against Staphylococcus aureus, the (UV-Vis) spectroscopy was achieved to confirm AgNPs which The UV-vis spectrum revealed the formation of wavelength peak for AgNPs is at (415 nm) and absorption peaks at (1.2 nm).

synthesis using  P. ostreatus fungus extract . All of S.aureus isolates were inhibited ,  decreased in number and desrtoyed in cell wall by AgNPs reduced by this fungus at different concentrations (50, 100, and 150) µg /ml . The largest inhibition zone of AgNPs was (4 cm) by a concentration of (150 µg /ml), whereas the lowest inhibition zone  was (1.5 cm) at the concentration (50 µg/ ml).