FACILE FABRICATION OF AG@SN3(PO4)-C3N4 AND ITS CYTOTOXICITY STUDIES ON ORAL CANCER

Abstract

Introduction

Ag@Sn₃(PO₄)₂–g-C₃N₄ is a hybrid material consisting of silver nanoparticles (Ag), tin phosphate , Sn₃(PO₄)₂–g-C₃N₄ and graphitic carbon nitride  C₃N₄.Silver nanoparticles,have demonstrated potential cytotoxic effects against various cancer cell lines.The phosphate ion is commonly found in various compounds, including salts and minerals. It plays a crucial role in biological systems, such as in DNA, RNA, and ATP (adenosine triphosphate), which is an energy-carrying molecule in cells.Graphitic carbon nitride (g-C₃N₄ ) is a two-dimensional material that has been explored for various applications, including anti-cancer activity.While g- C₃N₄ is  primarily known for its photocatalytic properties The development of nanomedicines, including silver nanoparticles, for cancer treatment is an active area of research, and scientists are exploring their potential in combination with other therapies for improved outcome’

Materials and methods

• calcination of Melamine——> Heat (500°C) under vacuum furnace ——> polymerization and condensation of the precursor ——> g-C₃N₄


• Silver nitrate + NABH4(reducing agent)+ (Stabilising agent ) ——> Thorough mixing to ensure uniform distribution.


• heat at 90c for 3 hrs ——>Ag nanoparticles


• Dissolve tin chloride in suitable solvent ——> tin chloride precursor


• Dissolve sodium phosphate in water


• Mix tin chloride precursor solution and the phosphate solution together in a sealed reaction vessel.


• Heat it up to 180 °C and maintain it for 16 hrs to promote the reaction and formation of tin phosphate


• Wash ppt with ethanol to remove impurities


• Dry at 150 °C for 12hrs


• Add Ag nanoparticles to the materials using ultra sonification for 3 h and then filtration, dry for 3hrs at 90 °C


• Ag@Sn₃(PO₄)₂–g-C₃N₄ is prepared

Results

The results showed that the Ag@Sn₃(PO₄)₂-C₃N₄  nanoparticles inhibited cell proliferation and induced cell death through apoptotic pathways.

Conclusion

The study suggests that tin phosphate nanoparticles have potential as a therapeutic agent for  cancer treatment. It is important to note that further research is required to fully understand the mechanisms underlying the anticancer properties of tin phosphate and to explore its potential in various cancer types. Overall, while the research on tin phosphate in anticancer applications is still in its early stages, these studies suggest its potential as a novel agent for cancer therapy.