IN VITRO SYNERGISTIC EFFECT OF ESSENTIAL OIL AGAINST PSEUDOMONAS AERUGINOSA.

Abstract

Background: Pseudomonas aeruginosa is one of the leading causes of nosocomial infections and is associated with high mortality. Infections caused by Pseudomonas are difficult to treat as the repertoire of useful antipseudomonal agents is limited. Moreover, Pseudomonas exhibits remarkable abilities to acquire resistance to these agents.

Method: In vitro synergistic effect of Cuminum cyminum (C. cyminum) essential oil (EO) against Pseudomonas aeruginosa PAO1. The C. cyminum EO was used for determination of minimum inhibitory activity (MIC) and synergistic effect of C. cyminum EO and chloramphenicol against P. aeruginosa PAO1.  

Results: The present study investigated the C. cyminum EO increasing the antibacterial efficacy at the level of 6.25%. Similarly, Chlorombinical alone inhibited the growth of bacteria at the concentration of 0.6mg/ml. The combination between C. cyminum EO (0.097%,v/v) and Chlormbinical (0.1mg/ml) effectively inhibited the growth of Pseudomonas. aeruginosa.

Conclusion: According to the study's findings, P. aeruginosa-caused bacterial infections can be treated with a combination of C. cyminum (EO) and antibiotics.