COMPARATIVE ANALYSIS OF CATALASE ENZYME PRODUCTION IN NORMAL HUMAN SALIVA

Abstract

Aim

The aim of this research is to explore and analyze the variation in catalase enzyme production within the saliva of healthy individuals and make comparisons regarding its activity levels among different subjects.

Introduction

Saliva contains a diverse array of enzymes, proteins, and other components that play important roles in maintaining oral health and aiding in the digestion process. Catalase is an enzyme that is found in various tissues and body fluids, including human saliva. Catalase acts to break down hydrogen peroxide into water (H20) and oxygen (02). This enzymatic reaction helps prevent the accumulation of hydrogen peroxide, which can be damaging to cells due to its oxidative properties.The production and activity of catalase in human saliva can vary among individuals due to genetic and environmental factors.

Methods

The salivary samples were collected by Spitting method. In this way, the patient was allowed to put it into a special sterile plastic tube (falcon). To 0.05 ml of sample 1.0 ml Citrate buffer and 0.45 ml of water is added. The Reaction is initiated by the addition of 0.5 ml of H2O2 and the reaction mixture is incubated at 37 °C for 1 min. Native gel electrophoresis was done in the same conditions as SDS-PAGE, but the polyacrylamide gel did not contain SDS and the migration was realized at 4°C.

Results

The results are interpreted based on the color change, which can be correlated with the catalase activity in the saliva sample. The higher the catalase activity, the more H2O2 is converted to O2 and H2O, resulting in a weaker color change in the final solution.Factors such as age, gender, and health conditions can influence catalase production. Environmental conditions, such as exposure to pollutants, dietary factors, and lifestyle choices, can impact catalase production. For example, smoking and alcohol consumption will decrease catalase activity.

Conclusion

The study concludes that the salivary catalase activity showed a minor variation among male and female samples. This investigation provides a new view on saliva enzymes that may be a useful tool for further investigation on the adaptive response, diet and for the evaluation of health and nutritional status of healthy and unhealthy persons.