ISOLATION OF PHOSPHORYLATED CHITOSAN FROM MARINE MOLLUSES SQUID PEN AND THEIR GROWTH OF INHIBITION IN DENTAL MICROBIAL PATHOGENS

Abstract

Introduction: The seafood processing industry produces large quantities of byproducts and discards such as heads, tails, skins, scales, viscera, backbones, and shells of marine organisms. Although these are waste residues, they still are an excellent source of lipids, proteins, pigments, and small molecules, and moreso a source of chitinous materials. One of the limitations in the use of chitin on a large-scale is its water insolubility, this is why water-soluble derivatives have been sought after.

Materials & methods : Chitosan is obtained from chitin by a process called deacetylation, and is converted into water-soluble form by phosphorylation. These chemical alterations produce a large variety of derivatives with several uses in a variety of industries.

Results: The phosphorylated chitosan derived from squid pen of  Loliolus investigatoris produced positive results on characterization using SEM, FT-IR and XRD comparing with standard chitosan. Also the antimicrobial activity was prominent against C. tropicolis, P. aeruginosa, and E. coli, but no significant results were recorded against S. mutans

Conclusion: The results showed positive results in the characterization of the phosphorylated chitosan and good antimicrobial activity against the dental pathogens.