EVALUATION AND EXTRACTION OF MELANIN PIGMENT FROM MARINE ACTINOBACTERIA AND THEIR INHIBITION OF STREPTOCOCCUS SP BIOFILM

Abstract

Aim: To evaluate and extract melanin pigment from actinobacteria and their inhibition of streptococcus sp biofilm

Introduction: Melanin is a biopolymeric pigment widely recognized for its role in providing coloration and protection in humans, animals, and microorganisms. Actinobacteria, a diverse group of bacteria, have been identified as a significant source of melanin production. Actinobacteria-derived melanin has garnered attention due to its wide range of biological activities, including antimicrobial, antioxidant, and anti-inflammatory properties.

Materials and methods: Isolation and Culture of the microorganism was done to isolate and analyze the bacteria. The Histamine analysis and the biofilm study helped us recognise the bacteria and isolate melanin pigment from the same. Further study of the melanin pigment was done using FTIR and Confocal imaging.

Results: It was observed that actinobacteria sps derived melanin pigment inhabit biofilms of the microorganism. Melanin pigment from the bacteria had proliferative activity on that of the streptococcus sps denoting it’s activity on the harmful bacteria.

Discussion:In contrast to regularly occurring actinobacteria like Streptomyces, unusual actinobacteria are rarely isolated. Due to incomplete knowledge of their growth needs, marine actinobacteria are equally challenging to culture in lab settings.