SYNERGISTIC OF SILIBIN AND RUTIN ENHANCES RUNX2 AND ALP EXPRESSION OR NOT

Abstract

A flavonoid substance called silibin is obtained from milk thistle (Silybum marianum). Studies have been done on it to see whether it has any anti-inflammatory, antioxidant, or hepatoprotective qualities. A flavonoid substance called rutin can be found in many fruits and vegetables.In some preclinical experiments, rutin has been shown to enhance osteoblastic activity, encourage bone formation, and prevent bone resorption. Normally, silibinin and rutin were bought separately, and 25 M of silibinin and 0.1 M of rutin was used to determine their synergistic effects (the amount was derived from linked papers). MG-63 cells were collected, and they were cultured for 3 days straight in both normal medium and osteogenic medium. Comparing Rutin, silibinin, and a combinational complex revealed fold alterations, and the expression of Runx2 and the ALP gene was also examined.Synergistic effects of silibin and Rutin enhances expression Runx2 and ALP especially in the osteogenic medium.