A NOVL DETECTION OF BACTERIAL COMMUNITY IN VAGINA BY METAGENOMIC AND MOLECULAR DETECTION OF A NEW LOCAL STRAIN OF MYCOPLASMA GENITALIUM

Abstract

Abstract

Metagenomic is a powerful genomic tool that allows the genomic analysis of microorganisms present in various environments. It enables the study of genetic information, including sequences and genomes of cultivable and non-culturable microorganisms that constitute the vast majority of organisms in many environments. In this study, 30 samples of vaginal swabs were taken by a specialized gynecologist using a sterile cotton and speculum swabs from women in Mosul city visiting Al-Khansa Teaching Hospital and Al-Batoul Hospital, and other outpatient clinics. Samples were collected from women who use Intrauterine Device (IUD) and have not used antibiotics, from the ages between 25-40 years and to determine the microbial environment by using  metagenomic analysis The results of Mitogenomics NGS showed a variation in the number of bacteria present in the environment of the vagina, as it was found that there are 14 different types of bacteria, some of which are considered normal flora or pathogenic, including Mycoplasma. PCR was also used to detect the presence of Mycoplasma by targeting a specific fragment from the 16SRNA gene. The results showed that 7 samples of vaginal swabs gave a positive result indicating a presence ratio of 23.3% for the presence of Mycoplasma. Our results indicate that the presence of IUD in women is often accompanied by genital tract infections, depending on personal hygiene. Our results show that molecular detection of Mycoplasma via specific primers in PCR is efficient in the direct detection in vaginal swabs.