OSTEOBLASTIC DIFFERENTIATION MARKER GENE EXPRESSION STATUS IN RESPONSE TO GINGEROL TREATMENT

Abstract

Introduction:

Using a bioactive compound named Gingerol which is derived from a traditional medicinal plant has a wide variety of therapeutic and medicinal use mainly in producing bone formation and osteoblastic marker gene expression.Mesenchymal stem cells(MSCs) helps in promoting the formation and mineralisation of bone.

Aim:

The aim of this study is to determine the Osteoblastic differentiation marker gene expression status in response to gingerol treatment and identify the osteogenic potential at non-toxic concentration of gingerol.

Materials and Method:

Materials used are the osteogenic cell line,gingerol and process it through MTT Assay,RT-PCR technique.

Result:

Three different analysis were taken I.e:Cytotoxicity assessment of Gingerol on osteoblast by MTT Assay 24 h,Col-1 gene expression in 7 days of osteogenic medium and Runx2 gene expression in 7 days of osteogenic medium,Results have shown that gingerol was found to be non-toxic concentration at 2.5 mM.

Conclusion:

From this preliminary study we can conclude that gingerol is non-toxic till 2.5mM and cells were able to survive in those concentration.So it promotes the osteoblastic regulation at specific concentration and upregulate the expression of COL 1 and Runx2 enzymes.It is speculated from this study that gingerol is used for used for bone related therapies.