INVESTIGATING THE ROLE OF RUTIN ON REGULATION OF MIRNA TARGETING RUNX2

Abstract

Introduction: The cells called osteoblasts are responsible for maintaining and repairing bone, and Runx2 is essential for osteoblast differentiation, function, and ultimately bone biology. MiRNAs are now regarded as one of the most effective regulators at the post-transcriptional level of gene expression, especially in light of the emergence of epigenesis and related regulatory mechanisms. Rutin can stimulate cell proliferation and osteogenic differentiation in vitro, which can effectively prevent and treat osteoporosis, according to research

Materials and Methods: Real time PCR, Western blot, Statistical analysis

Result: Rutin upregulates the expression of Runx2 and suppress the expression of miR-889-5p mRNA, there by promoting osteoblast differentiation and the related genes to be expressed in the osteoblast.

Conclusion: Rutin was able to promote osteoblastic differentiation by upregulating Runx2 and by directly reducing the level of microRNA 889 5p.