CYTOTOXIC EFFECTS OF CISPLATIN DERIVATIVES ON HUMAN CANCEROUS CELL LINES: A STUDY USING MTT ASSAY

Abstract

Abstract

The aim of this study was to investigate the cytotoxic effects of cisplatin derivatives on human cancerous cell lines using the MTT assay. Cell-based assays play a crucial role in assessing the direct cytotoxic effects of test molecules, which ultimately result in cell death. In this study, four different cell lines (HepG2, ZR-75, HL-60, and Daudi) were cultured in Dulbecco's Modified Eagle's medium (DMEM). After 24 hours of incubation, various concentrations (10-100 µL) of eight cisplatin derivatives were applied to determine their effects on cell proliferation. The MTT cell cytotoxicity assay was employed to evaluate the impact of these cisplatin derivatives on the different cell lines.

Results showed that in the HepG2 cell line, cisplatin derivatives 1100.002, 1100.004, 1100.006, 1100.008, and 1100.013 exhibited the highest survival percentages at a concentration of 80 µL. Cisplatin derivatives 1100.001 and 1100.003 demonstrated maximum survival percentages at concentrations of 50 µL and 90 µL, respectively. Compound 1100.009 displayed maximum survival percentages at two concentrations, namely 30 µL and 90 µL. In the ZR-75 cell line, cisplatin derivatives 1100.001, 1100.008, and 1100.013 exhibited the highest survival percentages at a concentration of 90 µL. Cisplatin derivatives 1100.002, 1100.003, and 1100.004 demonstrated maximum survival percentages at a concentration of 100 µL. Cisplatin derivatives 1100.006 and 1100.009 showed maximum survival percentages at 50 µL. Similarly, in the HL-60 cell line, cisplatin derivatives 1100.001 and 1100.008 displayed the highest survival percentages at a concentration of 60 µL. Cisplatin derivatives 1100.003 and 1100.009 showed maximum survival percentages at a concentration.