SEQUENCE, SECONDARY STRUCTURE, AND PHYLOGENETIC CONSERVATION OF MicroRNAs in MIR182 microRNA 182 [ HOMO SAPIENS (HUMAN) ]

Abstract

Introduction: The secondary structure and sequence of the miRNA are both conserved. Because it is conserved across species, we can infer that it serves a purpose and that it is probably involved in important regulatory functions. It is important to comprehend MIR182's sequence, secondary structure, and evolutionary conservation since doing so sheds light on its biological function and suggests potential implications for a number of biological processes and disorders.

Materials and methods: Selection of microRNA 182, sequence retrieved from NCBI, sequence analysis using mirBASE, conservative analysis using the Consurf server, secondary structure prediction of MIR182 by RNA fold server.

Results: The input sequence which is analyzed using mirBASE shows that the sequence is highly conserved as well as phylogenetically conserved and the secondary structure is predicted using the RNA fold server.

Discussion: The conservation of miR-182 across different species provides insights into its functional importance, miR-182 is highly conserved, it suggests that it plays crucial roles in biological processes across various organisms

Conclusion: The input sequence of the Homo sapiens region containing MIR182 was predominantly conserved.