RELATIONSHIP BETWEEN SERUM TESTOSTERONE LEVELS AND SEMINAL PLASMA MAGNESIUM AND ZINC LEVEL IN INFERTILE MEN WITH ASTHENOSPERMIA

Abstract

Abstract
Background: Many factors are affect negatively on male fertility one of them the chemical substances like Zinc and magnesium which may affect on testosterone level .The aim of this study is to measure the level of zinc and copper in  seminal plasma of infertile men with asthenospermia and their relation to testosterone level .
Methods: A sample of 100 men was involved in the study (50 infertile men with asthenospermia and 50 fertile men). The level of seminal plasma Zn and Mg in was measured by atomic absorption spectrophotometry . Fluorecare, China     Testosterone Kit was used to measure Testosterone level.
Results: the serum testosterone level in studied group was messured  , the group with asthenospermia had lower testosterone level than group with normospermia (p<0.001). The fertile male had higher Mg level than patients with asthenospermia  (p<0.001).Also , the male with normospermia had higher zinc level than patients with asthenospermia  (p<0.001). A strong positive correlation was found between testosterone levels and magnesium levels. Additionally, there was a significant positive correlation between testosterone levels and zinc levels (p>0.05).ROC curve was done , Zn had higher area under the curve , cut of value of 30 ppm give a sensitivity 95% and specificity 93% fallowed by Mg  , cut of value of 25 give a sensitivity 92% and specificity 90%.So both Zn and mg affect on sperm activity , lower level associated with asthenospermia 
Conclusion: Zn and Mg in seminal plasma may effect on sperm activity and positively related with testosterone level and may  help in explore the cause and treatment of infertile males.