ESTIMATION OF IL-6(174G>C) GENE EXPRESSION WITH CHRONIC HYPERTENSION PATIENTS IN IRAQI POPULATION

Abstract

Abstract

The study aimed to reveal the gene expression estimate for the IL gene (174 G>C) in patients with high blood pressure. The study included 50 blood samples, which 25 were from people suffering from chronic high blood pressure (different races) and their ages ranged between 35 and 68 years, and 25 healthy samples (control group), from different races, and their ages ranged between 17 and 17 years. 53 years old. Studied the estimation of gene expression of the IL-6(174G>C) gene using REAL-TIME-PCR technology (RT-PCR). The statistical results showed that there were highly significant differences between patients and the control group regarding the fold change in IL-6 gene expression. Values were expressed as mean ± standard deviation, which in the control group was (1 ± 0.000), while in the patient group it was (0.644 ± 0.153). The P value for the difference between groups is 0.024, indicating that there is a significant difference (S) between the two groups, as the patient group showed a lower change in IL-6 gene expression compared to the control group. A comparison was made between male and female patients in the study, including the number of cases in each group, the fold change in IL-6 gene expression, and the P value for the difference between groups. There were 14 male and 11 female patients in the study. The average fold change in IL-6 gene expression was (0.648 ± 0.169) in male patients and (0.638 ± 0.142) in female patients. The P value for the difference between male and female patients was 0.961, indicating that there was no statistically significant difference (NS) between male and female patients in the study. The spectrum changes in IL-6 gene expression and the value of the biological function (2-∆∆Ct) for each age group were also studied. In the group of ages under 45 years, the average change in IL-6 gene expression was 0.674 ± 0.192 and in the group of ages 45 to 55 years, the average change in IL-6 gene expression was 0.614 ± 0.132. In the group of ages over 55 years, the average change in IL-6 gene expression was 0.643 ± 0.123. The significance value was 0.975, which indicates that there are no significant differences between the different age groups.