EVALUATION OF THE ANTICANCER ACTIVITY OF HYDATID CYST FLUID AND PROTEINS EXPRESSION VARIANTS ON MCF-7 HUMAN BREAST CANCER CELL LINES

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Talib Waseen Hussein, Aymen Radhi Habeeb, Hayder A. Al-mandalawii

Abstract

Abstract:


Background:  In recent years, certain parasites, such as Echinococcus granulosus, have been thought of as agents of effect to immunological responses against both pathogens and tumors should be stimulated in order to avoid tumor escape and active monitoring by the tumor surveillance system. Therapeutics derived from Hydatid Cyst Fluid (HCF) have been show proven to be effective in the treatment of human cancer cell lines in experimental settings. The purpose of this study was to explore the influence of hydatid cyst fluid (HCF) crude on the cell activity of breast cancer (MCF-7) cell lines and the detection of gene expression. Materials and Methods: The fluid from the hydatid cyst was removed using a syringe and needle, then centrifuged at a speed of 2,000 g for two minutes. The supernatant was then air dried and kept at a temperature of 20 degrees Celsius to serve as a hydatid fluid antigen. MCF7 cancer cell lines were cultivated in RPMI-1640 media that was supplemented with 10% fetal bovine serum (FBS), 50 g/ml for ampicillin and streptomycin, and analyze the cytotoxic effect of HCF crude serial dilution (50, 25,  12.5, 6.25, and  3.125 g/ml) for 48 and 72 hours using an MTT test. Additionally, an RT-PCR test was utilized in order to identify the expression of several genes that are connected, such as EPCAM and KRT19. Results: The evaluations of the HCF crude Activity, the cells (MCF7) were treated for 48 and 72 hours using different concentrations for HCF. Also, our results showed up-regulation of KRT19 and done-regulation EPCAM genes.


Conclusion: According to the findings that we obtained in vitro, HCF crude is hazardous to MCF7 cell lines in a manner which is dependent upon both concentration and duration, as shown by an increase in the amount of apoptosis that is triggered by gene expression.


 

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How to Cite
Hayder A. Al-mandalawii, T. W. H. A. R. H. (2024). EVALUATION OF THE ANTICANCER ACTIVITY OF HYDATID CYST FLUID AND PROTEINS EXPRESSION VARIANTS ON MCF-7 HUMAN BREAST CANCER CELL LINES . Obstetrics and Gynaecology Forum, 34(3s), 1202–1208. Retrieved from http://obstetricsandgynaecologyforum.com/index.php/ogf/article/view/452
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