THE CYTOTOXICITY EFFECT OF SAFFRON AQUEOUS EXTRACT ON SKOV-3 CELL LINE

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Rusul Hammoodi Hasan Kabla, Prof. Dr. Jinan M. Abd-al-zahra

Abstract

Abstract

Cancer is the uncontrolled growth of abnormal cells in the body. Ovarian cancer (OC) is one type of cancer, and it is the third most common female cancer in the world (According to WHO in 2022). The aim of the current study was  to estimate  the effect of saffron aqueous extract on ovarian cancer cell lines (SKOV-3), the material and method was conducted  after maintaining of cell cultures in RPMI-1640 supplemented with 10% Fetal bovine serum, penicillin and streptomycin. Cells were passages using Trypsin-EDTA reseeded at 80% confluence twice a week, and incubated at 37 °C. To determine the cytotoxic effect of saffron, by MTT assay and acridine orange -ethidium bromid staining. The result of the study shows that there was morphological changes in SKOV-3 cell lines after being treated by the extract by using a fluorescent microscope like unregulated shape and low density. Moreover, the saffron has a highly cytotoxic effect against SKOV-3 cell line in addition it is can induce apoptosis in SKOV-3, which is caused by the induction of a programmed cell death mechanism that affects gene expression levels.


The conclusion of this study for using the saffron extract has high effect on the SKOV-3 and the effect increase with increasing the concentration of the extract and yield beneficial effects for individuals with ovarian cancer.

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How to Cite
Prof. Dr. Jinan M. Abd-al-zahra, R. H. H. K. (2024). THE CYTOTOXICITY EFFECT OF SAFFRON AQUEOUS EXTRACT ON SKOV-3 CELL LINE. Obstetrics and Gynaecology Forum, 34(3s), 2209–2214. Retrieved from http://obstetricsandgynaecologyforum.com/index.php/ogf/article/view/656
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