THE ANTICANCER EFFECT OF GRAVIOLA FRUIT EXTRACT IN COMPARISON WITH ANTICANCER-EFFECTIVE DRUGS

Abstract

Abstract

Malignant tumors are the world's second leading cause of mortality. Graviola, Soursop, or Guanabana, is a member of the Annonaceae family. It is native to the warmest tropical areas in South and North America. It is now widely distributed throughout tropical and sub-tropical parts of the world, including Nigeria, Malaysia, and India. Current chemotherapy treatments like 5-fluorouracil (5-FU) and Carboplatin often have limited success and fatal outcomes. Several studies have demonstrated the considerable antiproliferative effects of different extracts of Graviola and their separated acetogenins (AGEs) against various cancer cell lines; therefore, it may be a possibility as an alternative or adjuvant to chemotherapies. In this study, The GC-MS analysis shows that Many compounds with pharmaceutical properties were present in the methanolic extract as well as aqueous extract. The Kys-30 esophageal cancer cell line, SW480 colorectal cancer cell line, and Vero normal cells were treated with Graviola fruit extracts(GFE), chemotherapy drugs (5FU and Carboplatin), and GFES combined with chemotherapy drugs, then the cytotoxic effect of Graviola fruit extract (GFES) alone and in combination with drugs which Measured by MTT assay showed a growth-inhibitory effect as it reduced the viability of the cancer cell lines. The cytotoxicity assay results in the Vero cell line showed that the GFES had higher cell viability under the various tested concentrations. Therefore, the GFES is non-toxic to the treated Vero cell line. According to the results, Graviola extract is toxic to cancer cells, unlike normal cells, confirming its safety.