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Raneen Husham Shaalan, Amal Abdulwahid Mohammed , Muayad Sraibet Abbood




A seminal fluid analysis is responsible for providing information on the quantity, quality of the sperm. The CatSper cation channel is a specific calcium channel in sperm that is essential for the hyperactivation of sperm motility. Many males are infertile have down-regulation of CatSper3 gene expression. Therefore, this study hypothesized that the expression of CatSper3 gene may involve directly into various subnormal seminal fluid parameters.


The objective of this study is to evaluate the expression of the CatSper3 gene in both fertile and infertile males, and to determine the relationship between the level of gene expression, seminal fluid parameters.

Materials and Methods

In the resent study semen samples were composed from 50 males. The samples were categorized into five categories depending on particular sperm criteria obtained from typical examination of the seminal fluid: sperm concentration, progressive sperm motility, and normal in structure sperm. As follows: Group1: comprised 15 oligozoospermia males. Group2: comprised 3 teratozoospermia males. Group 3: comprised 7 asthenoteratozoospermia males. Group 4: comprised 16 asthenoligoteratozoospermia males. Group 5: comprised 9 normozoospermia males. A typical analysis of the seminal fluid was done following the rules given by the World Health Organization (2021). The samples were used to evaluate the sperm chromatin immaturity test by aniline blue. In the molecular method of this study, a quantitative expression of CatSper3 gene was executed by Real Time polymerase chain reaction. Then the percentage of sperm chromatin immaturity was related to the results of CatSper3 gene expression to show the influence of abnormal sperm chromatin immaturity on the result of seminal fluid analysis.


There was significant positive correlation between the fold of CatSper3 gene with sperm concentration (r=0.357; p=0.011), highly significant with total sperm count (r=0.398; p=.004), progressive motility (r=0.364; p=0.009), total motility (r=0.336; p=0.017).

The findings were revealed a significant reduction in the mean of gene expression fold (2-ΔCt) for CatSper3 gene in semen samples in, teratozoospermic and asthenozoospermic patient groups as following: [(teratozoospermic group: 0.162, AT group: 0.224, AOT group: 0.359)]. The lowest folding was detected in teratozoospermic group when in comparison to other subgroups of infertile men. Additional, oligozoospermic and teratozoospermic groups were showed downregulation of CatSper3 (0.234) while regulation expression of gene in normozoospermia group (1.000). Therefore, the results indicated a significant correlation between important factors comprised in the present study, such as semen parameters, with folding expression for CatSper3gene.


according to result the expression of CatSper3 gene correlate significantly with sperm concentration, motility and morphology, this study suggested that CatSper3 gene has influence in seminal fluid parameters more beyond its effects on sperm motility. Large study for each seminal fluid categories is needed.    

Article Details

How to Cite
Muayad Sraibet Abbood, R. H. S. A. A. M. ,. (2024). CATSPER3 GENE EXPRESSION AND SPERM PARAMETERS IN INFERTILE MEN. Obstetrics and Gynaecology Forum, 34(3s), 2042–2048. Retrieved from