HYPERBARIC OXYGEN TREATMENT REDUCES STRESS OXIDATIVE AND INFLAMMATION THROUGH SUPEROXIDE DISMUTASE AND INTERLEUKIN 1 Β IN ANIMAL MODELS EXPOSED TO MOTOR VEHICLE SMOKE

Abstract

The development of human civilization and the increasing variety and quantity of vehicles cause air pollution which is one of the biggest environmental risks for health. Exposure to air pollution is estimated to cause 7 million premature deaths each year. This study examined hyperbaric oxygen therapy (HBOT) strategies to reduce oxidative stress in experimental animals exposed to motor vehicle smoke through analysis of superoxide dismutase (SOD), interleukine 1b (IL-1b) and hemoglobin (Hb). Material and Methods: The research design used is true experimental research. The research design was a post-test only randomized control group design. 21 female rats (Rattus Norvegicus) aged 6-8 weeks weighing 180-200 grams were divided into 3 groups, the normal group (G0), the group exposed to vehicle smoke which were not given Hyperbaric oxygen therapy (HBOT) (G1) and the group exposed to vehicle smoke which were given HBOT (G2). HBOT is inhaling 100% O2 with a pressure of 1.7 ATA for 3x30 minutes with 5-minute intervals for 10 consecutive days. Oxidative stress was assessed based on the activity levels of the enzymes SOD, IL-1β and hemoglobin (Hb)as measured by the enzyme-linked sorbent assay (ELISA).There was a significant increase (p <0.05) in SOD and Hb levels, there was no significant decrease (p > 0.05) in IL-1β levels in the animal model group exposed to vehicle smoke that were given HBOT (G2) compared to with experimental animal models exposed to vehicle smoke that were not given HBOT (G1). Results: There was a significant increase (p <0.05) in SOD and Hb levels, there was no significant decrease (p > 0.05) in IL-1β levels in the animal model group exposed to vehicle smoke that were given HBOT (K2) compared to with experimental animal models exposed to vehicle smoke that were not given HBOT (K1). Conclusion: HBOT has an effect on reducing oxidative stress through increasing SOD enzyme activity and Hb levels and decreasing levels of the pro-inflammatory cytokine IL-1β in animal models exposed to vehicle smoke.