IDENTIFICATION OF B-CELL AND T-CELL SPECIFIC PEPTIDE VACCINE FOR MYCOBACTEROIDES ABSCESSUS

Abstract

Introduction- Illnesses of the skin and soft tissues, infections of the central nervous system, bacteremia, as well as ophthalmic and other illnesses are all brought on by a group of rapidly proliferating, multidrug-resistant, nontuberculous mycobacteria called the Mycobacterium abscessus complex. The current antimicrobial medications of preference for treatment include clarithromycin, amikacin, and cefoxitin. New treatment plans, however, are urgently required, as are quick and affordable diagnostic techniques, as well as steps to stop nosocomial transmission and epidemics. For many years, extremely effective methods for creating conventional vaccinations have been used to reduce infectious disease-related mortality and morbidity.

Materials and methods- Fasta sequences was used for B cell and T cell epitope prediction. Bepipred Linear Epitope Prediction 2.0 online server program was used to predict the B cell immunogenic epitopes. IEDB analysis resource program were used to analyse the results.

Results- 20 amino acids length B cell epitope and T cell epitope with the highest score were identified as the best candidates for peptide vaccine.

Discussion-Traditional vaccinations based on whole organisms or big proteins, however, are no longer recommended due to the unnecessary antigenic load that results in a lackluster protective immunity and exacerbates the problem by triggering reactogenic and/or allergic responses. On the other hand, peptide vaccines are a desirable alternative for triggering highly tailored immune responses and delaying the start of allergic and/or reactogenic events.

Conclusion- In this study we have identified B and T cell specific peptides against Mycobacteroides abscessus. They proved to have high efficacy against the bacteria as the amino acid length proved to be excellent.