PREPARATION OF AG@NI₃(PO₄)₂-G-C₃N₄ FOR ELECTROCHEMICAL BIOSENSING BOVINE SERUM ALBUMIN

Abstract

INTRODUCTION: The development of sensitive and selective biosensors has become a crucial area of research in fields, including healthcare, environmental monitoring, and food safety. Particularly electrochemical biosensors have many benefits, including high sensitivity, quick response, and ease of miniaturization. Composites based on nanomaterials have drawn a lot of attention recently for improving the functionality of electrochemical biosensors. One such material is Ag@Ni₃(PO₄)₂-g-C₃N₄ , which combines the special qualities of nickel phosphate, graphitic carbon nitride, and silver nanoparticles (AgNPs).

AIM: The aim of this study is to prepare the Ag@Ni₃(PO₄)₂-g-C₃N₄  nanocomposite and immobilize bovine serum albumin (BSA) on its surface for the development of an electrochemical biosensor.  The synthesized composite will be assessed for its potential use in the sensitive and specific detection of particular infections, contaminants, or biomarkers.

MATERIALS AND METHODS: Synthesis of  Ag@Ni₃(PO₄)₂-g-C₃N₄  Nanocomposite: The Ag@Ni₃(PO₄)₂-g-C₃N₄ nanocomposite is synthesized through a suitable method, such as a one-pot hydrothermal or co-precipitation process. The Ag nanoparticles are incorporated into the Ni₃(PO₄)₂ matrix, which is further combined with C₃N₄  to form the final nanocomposite.

RESULTS: Ag compound is highly sensitive, highly compatible, high in strength, and also highly selective. The composite material demonstrates positive results even at low concentrations of BSA, indicating its effectiveness for detecting and quantifying BSA in various samples.

CONCLUSION: The research on Ag@Ni₃(PO₄)₂-g-C₃N₄ biosensors for BSA detection provides a foundation for potential forensic applications in various areas of analysis and investigation