EVALUATION OF THE CERAMIDE LEVELS IN SERUM AND FOLLICULAR FLUID AND THEIR CORRELATION WITH OOCYTES AND EMBRYO QUALITY IN FRESH ICSI CYCLE

Abstract

Abstract

Background: Ceramide (CER) is a form of biologically active in the mitochondrial membrane and is essential for energy processing, cell division, and reproduction.

The aim of the study: to evaluate the relationship of Ceramide levels in follicular fluid and serum of intracytoplasmic sperm injection patients with oocyte characteristics, embryo grading, and pregnancy outcome.

Patients and Methods: The current study included 60 women. On the second day of the cycle, recombinant follicle-stimulating hormone (FSH) was administered to all of these women in preparation for ICSI. On the day of oocyte collection, samples of follicular fluid and serum were taken. These samples have their amounts of ceramide measured.

Result: The current study's pregnancy rate for all females was 30%.The mean age had a significant relationship between the two groups (P = < 0.05), and we found no difference in body mass index and the cause and type of infertility in pregnant and non-pregnant patients (all P > 0.05). There is a relationship between serum ceramide level, body mass index, ovarian reserve (AMH), follicular fluid ceramide level, and quality of eggs (P=0.009) and embryos. (p=0.049). It was revealed that serum ceramide (sCER) and follicular fluid ceramide (ffCER) levels were not related to pregnancy outcomes, (p > 0.05).

Conclusion: Serum ceramide level was positive correlation with Body mass index (BMI ) for women and Anti Mollerian Hormon (AMH). follicular fluid ceramide positive correlation with oocyte and embryos charactierstics.