THE IMPACT OF MIRNA-146A AND MIRNA-149 GENE POLYMORPHISM ON SUSCEPTIBILITY TO POLYCYSTIC OVARIAN SYNDROME (PCOS)

Abstract

Background: Polycystic ovary syndrome (PCOS) is considered one of the most prevalent disorders among females, with physiological and environmental factors. Nonetheless, a variety of hereditary illnesses are linked to this syndrome.
The Aim of study: This study is aimed to evaluate the association of miR-149 rs2292832 and miRNA-146a rs2910164 single nucleotide polymorphisms (SNPs) and PCOS
Patients and Methods: this is a case-control study which included 60 women with Polycystic Ovarian Syndrome (PCOS) and 60 aged-matched healthy women. Genomic DNA was extracted from whole blood, the gene fragments corresponding miRNA-146a and miRNA-149 SNPs were amplified with conventional PCR. genotyping was perfumed by (RFLP) Restriction fragment length polymorphism or amplification Refractory Mutation System-polymerase Chain Reaction (ARMS-PCR)
Results: The results indicate that the homozygous genotype (TT) of miR-149 rs2292832 was more frequent in patients (15%) Than Controls (5%) With a Significant difference (OR= 4.36, 95% CI=1.06-17.96, p=0.041). At allelic level, the mutant allele (T) was more frequent in patients than controls (39.17% versus 25.83%) with a significant difference (OR=1.85, 95%CI= 1.07- 3.2, p= 0.028). Similarly, the homozygous genotype (CC) of miRNA-146a rs2910164 was more frequent in patients (16.67%) than controls (3.33%) With a Significant difference (OR= 7.71, 95%CI=1.55-38.28, p=0.013). At allelic level, The mutant allele (C) was more frequent in patients than controls (38.33% versus 20.83%) with a Significant difference (OR=2.36, 95%CI= 1.33- 4.19, p= 0.003).