EXPLORING THE ROLE OF IL-10 IN DIABETES MELLITUS PATIENTS WITH ENTAMOEBA HISTOLYTICA INDUCED DIARRHEA

Abstract

Abstract

Background: A key immunoregulatory cytokine that predisposes amebic invasion to occur is interleukin-10. Intestinal amebiasis resistance depends on IL-10, and a lack of IL-10 increases a person's susceptibility to amebic infection. Because interleukin (IL-10) is crucial for preserving the mucosal barrier, it provided protection against infections caused by E. histolytica.

Objective: The goal of this study was to detection  of E. histolytica in Diabetes patients and compare immunological parameter IL-10 between Type 2 Diabetes mellitus patients and controls.

Methodology: 150 of participants (75 diabetic patients suffered from with diarrhea + 75 controls) were involved in this study and during the period from beginning of March 2023 to the end of Nov, 2023. Including people of two sexes as well as every generation. Each individual's identity, sex, date of birth, and place of residency were recorded using an individualized survey. At (Najaf Diabetes and Endocrinology Center, AL Hakeem Public Medical institution, AL Furat AL Awsat Medical institution and AL Sader Medical City) and all ages  took , whole participants in this examination topped up an individualized survey. Stool and blood samples were collected from all participants for detection of E.histolytica and measure IL-10 by ELISA technique.

Results: When comparing the means of IL-10 with a 95% confidence interval, the results showed that a statistically significant difference (P-value <0.0001) between the groups, with the mean concentration level of IL-10 in the patient groups being lower than in the control groups (29.808 ± 1.658 pg/ml and 43.529 ± 1.330 pg/ml, respectively). The average rank concentration of interleukin 10 peaked at its highest level (57.68 pg/ml) among a group of 28 diabetic patients who were infected with the parasite E.histolytica. Among the group of 48 diabetic patients not infected with parasite E.histolytica, the average level of interleukin 10 was at its lowest point (27.31 pg/ml). This variation had a p-value of 0.000, indicating statistical significance. The oldest age group (63-72 years) had the highest average rank for interleukin 10, reaching 55.00. Conversely, the youngest age group (3-12 years) had the lowest average rank for interleukin 10, reaching 10.29. There is no significant difference between males and females, or between urban and rural patients in the study group, as indicated by the Asymp. Sig. The (2-tailed) probabilities are 0.597 and 0.503, respectively. The average rank for IL-10 concentration levels in females (40.11) was slightly greater than in males (37.39). The average rank for IL-10 concentration levels was slightly higher in rural patients (40.38) compared to urban patients (36.98).

Conclusions: The IL-10 levels were found to be considerably lower in the patient group compared to the control group. The noticeable disparity in interleukin 10 concentration levels between diabetic patients with and without E.histolytica parasitic infection indicates a possible connection between parasite infection and elevated interleukin 10 levels in diabetic patients.