EFFECTS OF REMOVING FRAGMENTATION AT THE DAY TWO AND THREE OF DEVELOPED BLASTOMERE ON EMBRYO QUALITY IN ICSI CYCLES

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Faytha K.A, Al-Dujaily S.S
Al-Maliki R. Sh, Abbas S H

Abstract

Abstract


Background: During assisted reproductive technologies procedures, fragmentation and cell debris are considered important prognostic factors in the static morphologic assessment of human embryo quality, along with cell number, size, and symmetry. The goal of embryo grading at any stage is to select the embryos with the highest implantation potential to be transferred.


Objective: The present study was aimed to evaluate the effects of removing the fragmentations at day 2 and 3 from developed blastomere on embryo quality of in vitro fertilization/ intra-cytoplasmic sperm injection cycles.


Materials and Methods: This study was considered a retrospective analysis involving 40 couples enrolled and fit for intra cytoplasmic sperm injection (ICSI) program in High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University and Al-Zaitoon Specialized Fertility Center through the period from November 2023 till April 2024. All developed embryos which had over >25% fragmentation (grade 3 or 4) on day 2 and 3 of the - cleavage stage were micromanipulated to eliminate fragmentation. Embryos were divided into two groups depending on the response to cleavage development after removal of fragmentation;1- positive developed embryos group (n=20) and 2- negative embryo non –developed group (n=20). Microsurgical fragment removal was performed by micromanipulation with later-assisted hatching and a handmade suction micropipette that had an outer diameter of 30 µm. All the results of ICSI program was measured and reported.


Results: Insignificant increase of embryos Grade I and II at day 2 after removal of fragmentations with decline in Grade III and Grade IV embryos were observed. There was a significant increase in Grade III embryos (P=0.047) in negative non-developed embryos compared to positive developed embryos after fragmentation removal at day 3.


Conclusions: It is concluded from the results of current study that elimination of fragmentations from cleavage embryos at day 2 and 3 resulted from ICSI program had a positive effect on blastomere quality and embryonic development. Because this study the first Iraqi trail, it is recommended to transfer and/ or cryopreserve these embryos in future to obtain higher percentage of pregnancy rates.


 

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How to Cite
Al-Dujaily S.S, F. K., & Abbas S H, A.-M. R. S. (2024). EFFECTS OF REMOVING FRAGMENTATION AT THE DAY TWO AND THREE OF DEVELOPED BLASTOMERE ON EMBRYO QUALITY IN ICSI CYCLES. Obstetrics and Gynaecology Forum, 34(3s), 1720–1726. Retrieved from https://obstetricsandgynaecologyforum.com/index.php/ogf/article/view/584
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