DETERMINATION OF ALPHA GLUCOSIDASE INHIBITION ACTIVITY OF ODINA WODIER BARK

Abstract

INTRODUCTION : Diabetes is a global health concern affecting millions of people worldwide.It is a chronic metabolic disorder characterized by high blood sugar levels (hyperglycemia) resulting from the body's inability to produce enough insulin or effectively utilize it. alpha glucosidase is an enzyme found in the small intestine that breaks down complex carbohydrates into simple sugars, which are then absorbed into the bloodstream. Inhibition of this enzyme can help regulate blood sugar levels and manage conditions like diabetes.Odina wodier, commonly known as the Indian silver oak, is a tree species found in various parts of Asia, including India. It is known for its medicinal properties and has been traditionally used in Ayurvedic and folk medicine for the treatment of various ailments.

AIM: The Aim of this study is to examine the alpha glucosidase inhibitory activity of Odina wodier bark and evaluate its potential as a natural inhibitor for treating metabolic illnesses such as diabetes.

OBJECTIVES: The objective of this study is to examine the alpha-glucosidase inhibitory activity of Odina wodier bark in order to determine its potential as a complementary treatment for controlling blood sugar.

MATERIAL AND METHODS: To determine the binding energy of putative MMP8 inhibitors, docking analysis was used. Based on a thorough review of the literature, phytochemicals were chosen, and the PubChem database was used to retrieve their chemical compositions. As a reference, the three-dimensional structure of glucosidase (PDB ID: 3L4Y) was used. Rigid docking analysis was performed using Hex 8.0.0, and Pymol was used to display the docked complexes. A greater connection between the ligand and receptor, potentially increasing receptor activity, was indicated by a more negative E-total value.

DISCUSSION: The strong alpha-glucosidase inhibition found in the bark of Odina wodier suggests that it may be used as a natural diabetes treatment to slow the digestion of carbohydrates and lower blood sugar levels.

RESULTS: The study found that the bark of Odina wodier has considerable alpha-glucosidase inhibitory activity, suggesting that it may be useful as a natural diabetes treatment by controlling blood sugar levels by blocking the digestion of carbohydrates.

CONCLUSION: Odina wordier bark exhibited inhibitory effects. Visualizing the protein-ligand interaction using PyMOL revealed strong interactions with a more negative E-total value in Hexdocking server 8.0, suggesting receptor activation.