URINARY MICRORNAS AS INDICATOR OF BLADDER CANCER AND INSPECTION OF THE MECHANISMS OF (MIRNA-93-5P AND MIRNA-516A-5P) IN IRAQI PATIENTS

Abstract

Abstract

Objective: This study's objective was the possibility of using urine-derived miRNAs (miR-516a-5p and miR-93-5p) as good indicators for detecting bladder cancer.

Methods: The study was conducted from November 2022 to May 2023 in Ghazi AL_ Hariri Hospital of the Medical City (Baghdad/ Iraq). We studied a total of 45 individuals (25 BC and 20 healthy controls), using RT-q PCR tests.

Results: This study's results showed that the gene expression of miR-93-5p and miR-516a-5p was discovered by RT-q PCR, It was the miR-516a-5p and miR-93-5p were upregulated in bladder cancer patients compared to controls. The level of miR-93-5p was significantly different in the patients of BC compared to the control (P < 0.001)*, but the level of miR-516a-5p showed no significant differences compared to the control (P > 0.001). The AUC values of miR-93-5p were 0.772 (95 % CI: 0.636–0.908) and miR-516a-5p were 0.476 (95 % CI: 0.301–0.651). The sensitivity and specificity were (52 and 95%), (55 and 50%) respectively. The AUCs of miR-516a-5p and miR-93-5p did not differ significantly (p > 0.05).

Conclusion:  Conclusion: miR-93-5p and miR-516a-5p are not good biomarkers for the diagnosis of bladder cancer. These findings imply that these urine-derived miRNAs may be play important role for the etiology and progression of BC. However, further independent more independent research is required to validate our findings and demonstrate the potential clinical utility of our miRNA.