ASSESSMENT OF PREGNANCY IN ESTRUS SYNCHRONIZED IRAQI EWES BY APPLICATION DIFFERENT DIAGNOSTIC METHODS AND A COMPARISON BETWEEN THEM

Abstract

Abstract

            The aim of current study was to evaluate and compare sensitivity , specificity and accuracy of pregnancy associated glycoprotein (PAG- ELISA), progesterone (P4-ELISA)  and ultrasonography testes for distinguish between pregnant and non pregnant Iraqi ewes at day 30 an 60 of pregnancy.

            Eighteen synchronized Iraqi cross breed ewes  were scanned by trasrectal ultrasonography  (6.5 MHs liner probe ) at day 30 of pregnancy and transabdominal  ultrasonography (3.5 MHs sector probe) at day 60 of pregnancy. After each scan blood were collected  from jugular vein to confirm the values of ovain pregnancy associated glycoprotein (ovPAG) and progesterone( P4) by  ELISA. The distinguish value for detecting pregnancy  by  sheep PAG ELISA kit, sheep P4 ELISA kit and ultrasonography, based on parturition data. The predictive value of positive and negative test, accuracy, sensitivity and specificity of the PAG- ELISA were  100%, 100%, 100%, 100%and 100% on both 30 and 60 of pregnancy respectively and  the predictive value of positive and negative test, accuracy, sensitivity and specificity of the P4- ELISA were  100%, 100%, 100%, 100%and 100% on both 30 and 60 of pregnancy respectively , while the predictive value of positive and negative test, accuracy, sensitivity and specificity of ultrasonography  were 100%, 62.5%, 83.3%, 76.9% and 100% at day 30 and 100%, 100%, 100%, 100%and 100% at day 60 of pregnancy respectively.  It can be concluded  that ovine pregnancy  is reliably diagnosed at day 30 after mating by using  sheep PAG- ELISA  and  sheep P4- ELISA, but PAG- ELISA consider the most reliable because to P4 from CL of pregnancy or from CL of other physiological or pathological sources.